8/31/2023 0 Comments Inbound call center agent![]() They no longer want to settle for a poor customer service experience. What do these stats mean?Īlmost every customer service and call center statistic above shows that customers have high service standards. 85% of customer service executives say that customers are more likely to share positive or negative experiences now than in the past. ![]() 48% of consumers switched brands due to better customer service by the other company.ġ3. 83% of people prefer to speak to just one person to resolve any complex customer issue.ġ2. 53% think most customer support interactions are fragmented since they need to interact with executives over multiple channels or calls.ġ1. 59% of them prefer communicating their questions and issues via live calls.ġ0. ![]() 70% of customers found it easier to reach a real person during the pandemic’s beginning than now.ĩ. 44% of people report being annoyed or irritated when kept on hold for 5 to 15 minutes.Ĩ. 90% of them say that an immediate response is very important when they have a customer service question.ħ. 94% of customers say that positive service experiences make them more likely to purchase again.Ħ. 88% believe trust is more important in periods of change, like adopting new technologies or customer service processes.ĥ. 96% of consumers say excellent customer service builds trust.Ĥ. 88% feel that the experience a company provides is as important as its product or services.ģ. 91% of consumers reported poor customer service in 2021.Ģ. Here are call center statistics on customer experience and expectations based on Replicant’s 2021 Survey, Hubspot’s 2022 Report, and Salesforce’s 2022 Report:ġ. Knowing what your customers want helps you satisfy their needs immediately and give them a positive experience. They also give you a good idea of what the future holds for call centers. These customer service statistics highlight how large and small businesses adapt to modern needs. You’ll be able to future-proof your work processes. Plus, regularly paying attention to every relevant call center statistic will ensure you’re always ahead of the curve and your competitors. It enables your customer service team to keep up with evolving customer needs and deliver great customer service.Įach statistic offers a glimpse into what you could improve. You can identify customer expectations and structure your services accordingly. Tracking call center statistics is a good way to keep up with changing industry standards. Why you need to track call center statistics We’ll show you how each KPI can affect your service experience and what you need to do to remain productive. We’ll also cover the 10 metrics you need to track to ensure that your call center operation runs smoothly. In this article, we’ll highlight the top 41 call center stats from 2022 that you need to monitor in 2023. Keeping track of call center statistics and performance metrics is key to improving productivity, profitability, and customer satisfaction.
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![]() ![]() Trixy’s drag style is eclectic, but whether silly or fierce, there’s glitter: “I just want to shine when the light hits me.” Jacob started about six months later, in a white Marilyn Monroe dress at an amateur pageant in 2014. In their teens, Joshua was the first to turn to drag. Trixy Valentine, aka Jacob Kelley, drag queen ![]() Kelley is an LGBTQ+ activist and educator with a masters in human sexuality. Trixy Valentine, aka Jacob Kelley, performs to “The Devil Went Down to Georgia” by the Charlie Daniels Band during the “Spring Fever Drag Brunch,” Sunday, March 26, 2023, at the Kulpmont Winery in Kulpmont, Pa. I knew I was attracted to boys and loved all things feminine! I kept this side of me to myself and my best friends growing up, who really didn’t see anything strange about it.” “I still was not out at this point,” Alexus says. She soon entered her first drag performance in the small Pennsylvania coal town of Weishample. “I was just having a day!”Īlexus hit high school and upped her Halloween game. “I had no idea that it was drag or gay,” she says. With Cyndi Lauper and the Pointer Sisters blasting, she would wrap herself in knitted blankets to lip-sync and dance for her family. (AP Photo/Carolyn Kaster)ĭaniels’ first memory is of her great-grandmother’s jewelry box. Daniels, the drag family matriarch, was the child of a coal miner and textile worker who was “born with a female spirit.” She works at the local hospital as an MRI aide tech. Among them:ĭrag queen Alexus Daniels lip-syncs to “Heart of Glass” by Blondie as she sits on the lap of a smiling and consenting audience member and others take photos during the “Daniels Family Values” drag show at the Heritage Restaurant in Shamokin, Pa., Saturday, April 16, 2022. The Associated Press followed the Daniels family for more than a year. “And drag now can be a place or a thing to show people like you that you don’t have to go to the cities. because there’s no one like us here, there’s no one that can understand us. “There was a curse being a queer person in a rural town - the curse is that we’ll move. “I never had a person like me growing up,” Trixy said, “and now I get to be that for everyone else. Soon that seaman, Petty Officer 1st Class Joshua Kelley, who just reenlisted, moves from a base in Norfolk, Virginia, to one in Spain, with plans to pack a wig “and maybe one or two cute outfits but nothing over the top” for Harpy-style shore leave.Īpart from the twins, the drag performers in this circle are family by choice, not genes. Navy sailor who’s had three deployments on the aircraft carrier USS Ronald Reagan. Jacob Kelley, who performs as drag queen Trixy Valentine, is an LGBTQ+ activist and educator with a master’s in human sexuality. Not plain, not simple, but also bedrock, rising above ground only in culturally adventurous cities. The other, back to before Shakespearian times, is drag, a loud, proud and seismically flamboyant artistic expression of gender fluidity. One tradition is the view of family as mom, dad and kids, plain and simple. Here two very old traditions mingle - and mostly happily, it seems, in contrast to the fierce political winds ripping at drag performances and the broader rights of LGBTQ+ people in red states from Utah and Texas to Tennessee and Florida. In a string of towns running along a coal seam, the sparkle of small-town drag queens and kings colors a way of life rooted in soot, family and a conservative understanding of the world. Or they’re reading in gardens to children dressed in their Sunday best - Dolly Parton’s “Coat of Many Colors” is a favorite book for performers and kids alike. Or they’re lighting up local blue-collar bars and restaurants with Mimosas & Heels Drag Brunches for bridal parties, members of the military, families and friends. They’re hosting sold-out bingo fundraisers at the Nescopeck Township Volunteer Fire Co.'s social hall, packed with people of all ages howling with laughter and singing along. (AP) - Deep in Pennsylvania coal country, the Daniels drag family is up to some sort of exuberance almost every weekend. 8/31/2023 0 Comments Joplin highschool![]() Another attempt was made to revive the historic structure this year. Since then, numerous attempts to revitalize the building have failed. Ownership changed hands several times before the Missouri Department of Natural Resources (MoDNR) took over the building following foreclosure in 1989. In 1973, the depot was listed on the National Register of Historic Places, but it soon was abandoned and began to decay. The depot served as a community centerpiece, operating two railroad services-the Kansas City Southern Railway and the Missouri-Kansas-Texas Railroad-until 1969, when the station ceased operation. ft.," the January 1912 edition of Popular Mechanics reported. The train sheds and platforms cover 25,000 sq. The center portion of the station, built in the old Roman type of architecture, is two stories high. "A striking example of what may be done with the waste of zinc and lead mines has been demonstrated in the construction of the new union station at Joplin, Mo., the concrete of which is largely made up of flint and limestone tailing taken from the waste piles of mines in the district. The Joplin Union Train Depot in March 2023. Curtis' techniques were so innovative that the building was featured in an edition of Popular Mechanics. ![]() Curtis himself was an early pioneer of glass curtain walls and utilized mining waste in the building's concrete. Designed by Canadian-born, Kansas City architect Louis Curtis, the depot was one of the first fireproof reinforced-concrete structures built in the country. This spring, four faculty members-led by Pat Crawford, professor and director of the School of Design, and Elizabeth Tofte, assistant professor of landscape architecture-and nine students traveled to Joplin, Missouri, to take on a significant community visioning project that could bring a dilapidated train station back to life.īuilt in the early 20th century, the Joplin Union Train Depot operated for nearly 60 years in downtown Joplin. That's the question faculty members and students in South Dakota State University's School of Design set out to answer. What should be done about old, abandoned and decaying buildings? The team, composed of faculty members and students from South Dakota State University's School of Design, near the entrance of the Joplin Union Train Depot in March 2023. ![]() ![]() The world's first nuclear-powered submarine marked another important milestone in underwater technology. Image courtesy of Flickr user Andrew Kalat The sonar room, which visitors can pop into, with displays that helped the crew detect contacts, avoid collision and follow objects. Because the Hunley is so old and still being studied, however, visitors can’t enter inside. The sub eventually went on display in North Charleston, where those who visit can learn not only about the vessel itself and the stories of its crew but about the technology used to recover it. The vessel included technology they had not expected to find, such as a flywheel designed to act as a break for the propeller-an advanced feature for its time. Luckily, the sub was in remarkable shape, Hambrick explained, because it had been buried relatively quickly in a layer of silt that protected it from salt erosion. In August 2000, the team dredged up the Hunley and found a much more impressive machine than they’d imagined rotting beneath the sea. “Nobody has raised an entire ship before, so they had to go about figuring out how to do it,” Sherry Hambrick, who works for the nonprofit that now displays and preserves the Hunley, told. But to actually raise the sub from the ocean required a whole new kind of effort. Still, no dice. Finally, on May 3, 1995-20 years ago this month-a team of archeologists funded by adventure novelist Clive Cussler finally found it. Barnum even offered a reward of $100,000. The Hunley earned that inaugural honor during the Civil War, when it was built by the Confederate side and used in 1864 to attack the USS Housatonic with a 135-pound torpedo. The Hunley itself sank a little while later, under mysterious circumstances. For years afterward, explorers and treasure-seekers tried to locate the boat, and P.T. Hunleyin North Charleston, South Carolina. To see the first combat submarine ever to sink an enemy ship-a big milestone in the history of warfare- visit the H. ![]() Hunley, North Charleston, South CarolinaĪ drawing of the Hunley, showing its tightly packed crew members. And if you want to ride in one yourself, there are even some tourist submersibles that can take you underwater. A host of famous submarines are on display around the world, ready for visitors to explore. ![]() According to the company that sells it, the orca-styled submersible can be yours for $90,000.įor those who don’t want to join the Navy-or don’t have $90,000 lying around-there’s still hope for adventure. Today, submarines can be hulking-such as the 574-foot-long Soviet Typhoon-or sleek and miniature, like this two-person sub that looks and moves remarkably like a killer whale. Still, only one person could squeeze into Bushnell’s ship, which Parrish writes looked like two bathtubs clamped together, or like the shell of a strange oyster. Other inventors continued to make rudimentary submersibles until finally, in 1775, a man named David Bushnell created a machine that fits Parrish’s definition of a submarine: a vessel that can propel itself on water but also underneath it, and that can sink and rise again at will. This craft worked 15 feet below the surface of the Thames River, and according to Tom Parrish, author of The Submarine, even King James I visited onboard, despite the risk of drowning. He wouldn’t share them, he said, “because of the evil nature of men who practice assassination at the bottom of the sea.”ĭa Vinci never constructed his machine, as far as we know, and it wasn’t until about 1723 that a submersible came to life. Famed inventor Leonardo da Vinci, who died in 1519, had an idea for a submersible vessel but kept his sketches a secret. The idea of a ship that can travel underwater has been around far longer than the technology to make it possible. 8/31/2023 0 Comments Purple lightning backgroundWith the sun still out, beachgoers were making the most of the day while they could. There were still plenty of folks that hit the water to cool off and have a good time with the lifeguards keeping a close eye on them. “The waves aren’t strong enough to intimidate people, but it starts to form those rip currents that are really worrisome.” “It’s nothing crazy but it’s that surf right around two, two and a half foot where it really makes some of our rip currents the worst,” said Beach Safety Chief for the city of Orange Beach, Brett Lesinger. The surf wasn’t what most would consider big, but beach safety officials posted red flags because rip currents were still a concern. 431,932 lightning background stock photos, vectors, and illustrations are available royalty-free. ![]() The conditions Monday morning gave hope for a promising beach day in Orange Beach. All of these Purple Lightning background and pictures are for free download on Pngtree. Add to Likebox 140596175 - Purple line High voltage sign icon isolated on blue. Pngtree provides you with 135 free hd Purple Lightning background images, photos, banners and wallpaper. Blue Battery icon isolated on purple background. Lightning was also an issue for beachgoers when a lightning storm cleared the beach around lunchtime. Affordable and search from millions of royalty free images, photos and vectors. The elevated risk of rip currents wasn’t the only danger. ![]() ( WALA) - Red flags were flying on Baldwin County beaches Monday, July 10, 2023. Download and use 10,000+ Purple Lightning stock photos for free. 8/31/2023 0 Comments Tico sports![]() Explore our collection and find the perfect outfit for your next adventure. Whether you're camping, cycling, skiing, or just enjoying the great outdoors, our clothing is designed to provide protection and comfort in any environment. Tiso offers a wide selection of durable and comfortable outdoor clothing for a variety of activities. Trust us to supply you with the gear you need to make the most of your skiing adventures. Browse our collection of skiing equipment and clothing, including skis, boots, jackets, and pants. Tiso is your source for top-performing skiing gear. With a crew consisting of David Andrade (play-by-play), Allen Gutierrez (analyst) and Victor Flores (producer), Tico Sports will broadcast home and away games on Radio La Mera Mera 1050 AM and. Whether you're commuting, competing, or exploring, Tiso has the gear you need to make the most of your ride. Tico Sports se asocia con equipos profesionales y universitarios para impulsar la activación con el mercado latino de alto crecimiento. Choose from our extensive collection of cycling apparel and accessories, including breathable jerseys and shorts, durable helmets, and a wide range of bikes. Tico Sports, airs every Jaguars game on WBOB 600 AM and 101.1 FM, the team’s Jacksonville flagship Spanish radio partner, and on 107.3 Solos Exitos in Orlando. ![]() Our wide selection of high-quality camping equipment includes tents, sleeping bags, backpacks, and outdoor clothing for men, women, and kids Trust us for the best gear to make your next camping experience a success.Ĭyclists, look no further than Tiso for all your gear needs. They will use these skills to positively affect the student body at Kansas State, the Manhattan community, and beyond.Find everything you need for your camping trip at Tiso. The Kansas City Royals announced they will continue their partnership with Tico Sports to produce 14 Spanish language broadcasts this season. Wildcat 91.9 will work to foster the unique perspective and skills of all involved students. Students are provided the unique opportunity to learn professional broadcasting skills in an engaging, hands-on environment. Get the latest Champions League 2022-2023 football results, fixtures and exclusive video highlights from Yahoo Sports including live scores, match stats and team news. Wildcat 91.9-FM provides original and diverse programming that aims to educate and entertain its listeners. Wildcat 91.9-FM is a non-profit, student run radio station service Kansas State University and the city of Manhattan. Teaching and creating more media in Spanish."įollow Wildcat 91.9's Cardenas and Martinez's Sugar Bowl adventures with Tico Sports through Wildcat 91.9’s social media. One of their branches, Tico Sports, broadcasts Kansas City Chiefs games in Spanish, and over the last few years, the company and their audience have seen tremendous growth. Company President CiCi Rojas said the sports productions are reaching an ever-growing audience. "Even though the class was online, and Fernanda was taking it from Mexico, I have worked with so many media producers that I knew right away Fernanda had the chops to run a show. Established in 2016, Tico Sports is an arm of Tico Productions, a Kansas City marketing agency. The other recommendation that Punnett had in mind was junior Fernanda Martinez, a recent transfer to the school, whom Punnett had taught in a summer writing class. I have a couple other support people for you, too." ![]() "He is the number one prospect you need to talk to for Tico. For the first time in K-State Athletics history a sporting event will have a dedicated Spanish language broadcast as K-State Athletics and Tico Sports, a division of Tico Productions, LLC, have announced a partnership to become the official provider of Spanish broadcasts for the next three K-State Football games. "I told her right away, 'I know a guy,’" Punnett recalled. Miller School of Media and Communication professor and Wildcat 91.9 faculty advisor Ian Punnett in search of bilingual students who might be interested in participating in some capacity in its first ever Spanish broadcasts of Kansas State Wildcats football. Before he even had a chance to see the inside of Bill Snyder Family Stadium, however, a representative of Tico Sports reached out to A.Q. allow us to have logistics extensive commercial and sporting events such as the. 5 Alabama for Tico Sports, a national Spanish language broadcast network.Īs an incoming freshman earlier this year, Cardenas joined Wildcat 91.9 with the hopes of calling Wildcat games in English. All games are streamed on the KC Chiefs Mobile App (Restrictions Apply), Tico Sports website (PC or Laptop) and the NFL Game Pass (Membership Required). ATPIs 101 years of experience alongside athletes, international sports. 9 Kansas State's Allstate Sugar Bowl appearance against No. ![]() 20, 2022 - Wildcat 91.9 (KSDB-FM) sports commentator and broadcaster J.P. K-State freshman chosen for national Sugar Bowl play-by-play coverageĭec. 8/31/2023 0 Comments Sailor moon helios figure art![]() It is /generally/ of the moderator's opinion that if something is Sailor Moon related- then upvoting and downvoting by the community should determine whether it remains up top or not- which includes fan art, memes and captioned pictures.***īootlegs are allowed to be posted, as long as it's made clear that they are bootlegs and not being passed off as official merchandise. Anything else reported as 'spam' will be handled on a case by case basis as decided by the moderators and will be removed if deemed as spam. Spam on /r/sailormoon is defined as any link not relevant to Sailor Moon, or very low effort posts in general. Advertisement for personal projects you just want traffic to- such as linking to your DeviantArt, A Discord You Run, a Forum you want to invite people to or show off a piece of art or writing you did, are all allowed. If you wish to advertise that on Reddit, or target this subreddit, you will need to go through. This includes websites such as Etsy, Redbubble, Kickstarters and similar websites where the goal is to sell your product or sell your service. Advertising for Products or Services are not allowed. #4.) Referral Links / Advertising / Selling Do not post direct links to subs or dubs. This extends to both visual and written works.ĭon't post or ask about direct links to/or mention torrents/unofficial download or streaming websites. Since the majority of the characters in Sailor Moon could be considered as such- the main 'heroic' cast for example- anything that could be perceived as hentai or pornography or contains nudity is banned, as is anything that could be perceived as 'suggestive'- 'Come hither' poses, Pictures where gratuitous exposed chest/cleavage feature or are the focus of the picture for a couple small examples. Reddit has a policy against underage characters, or characters that could be considered underage, in sexual or suggestive situations that can be read here: ( ). 'But he did it first' is not an appropriate defense. Remember, treat others as you want to be treated. Most certainly don't use racial slurs, sexist slurs or put anyone down or harass anyone. If you have critique on someone's fanworks- be productive about it. Please hover over the rules below for more information. r/sailormoon has a few rules we'd like you to follow while posting and participating here. Welcome to /r/SailorMoon, Reddit's unoffical Sailor Moon Subreddit! Please feel free to post Sailor Moon relevant articles, news, fan art- or anything else related to the Sailor Senshi. 8/30/2023 0 Comments Double sided velcro walmartFor certain DIY projects, however, you may choose to buy a brown or yellow color to effortlessly match the wood finishes you’re working with. Colorįor most needs, clear tape works best as it’s barely visible on most surfaces. ![]() There’s no use in saving some tape if it means your expensive wall clock might fall off just because you used a recycled piece of double-sided tape with compromised adhesion. Keep in mind, however, that the piece of tape will lose some degree of adhesion upon each use, so it’s not advisable for heavy-duty needs. Reusabilityĭouble-sided tapes that are designed to be easier to remove, can often also be reused several times. There also won’t be any sticky residue upon removal, and that’s a blessing since that stuff is extremely hard to clean. It’s specially designed to have less adhesion and remove easier, which means fewer chances of ruining or tearing the surface. If you plan on removing the tape a few weeks or months down the line, we suggest going with temporary double-sided tape. For tidier results, it’s recommended to warm the area up before removing the tape as it allows you to peel it off smoother and in one piece. Premium ones don’t usually leave a messy-looking trail of residue behind upon removal. The quality of double-sided tape doesn’t only show in how well it sticks, but also how flawlessly it comes off. In the case of your car’s windshield or rear glass, be sure to get high-quality transparent tape that has no noticeable effect on visibility. Transparent tape is a must-have for glass repairs, window maintenance, car light restoration, and much more. The latter, however, might stick out and become visible on the edges, in which case you’d be better off with a transparent wide double-sided tape to ensure sufficient strength for heavy-duty projects without sacrificing aesthetics. Just make sure it doesn’t stick out.Īs a general rule of thumb, we’d recommend going with narrow tapes for light-duty needs and wider ones for sticking together or holding up heavier objects. For large (and heavy) pieces, however, you might need wider tape for covering more surface area with just one strip. Narrow double-sided tape can easily hide away between two surfaces which helps keep your DIY projects neat. Just like the strength of your tape roll, you’ve also got to keep its length and width in mind to make sure it’s sufficient for your particular needs. You do sacrifice some stability when you choose not to drill or nail your walls, but you also get a lot of convenience and ease of use in return! Dimensions With that said, none will have the same level of strength that you get with screws or nails, but it’ll still be more than you need for most needs. Many regular ones claim to hold 15 to 20 pounds of weight, but the strongest heavy-duty options can even make promises as bold as 30 pounds! Important Qualities to Consider Strengthĭepending on what you plan on using for your double-sided tape, strength is a key metric to consider. For household use, it’s the most common type out there and probably the right choice for most household and DIY needs. Tissue tape shares many similarities with light-duty cellophane tape, with the only significant difference being that it is double-sided. This type is also called foam tape, and it’s generally thicker than average to offer better insulation. Being waterproof, it’s the ideal variation of double-sided tape for outdoor usage. It’s made out of strong chemicals and provides an (almost) permanent bond. This double-sided tape is the most commonly used type for industrial uses. It’s an easy, effective, and damage-free way to get the job done. Here’s a quick rundown: FloorĪs you can judge by the name, floor double-sided tape is meant for keeping rugs or mats in place when placed on tiles or hardwood. Not all of them are created equal, and some are better suited for certain applications. Here’s everything you need to pay attention to! Typesįirstly, let’s go over the several types of double-sided tapes available in the market. ![]() ![]() We’ve created this well-researched buyer’s guide to help you navigate the market like a pro. The market offers these in different types, widths, lengths, colors, and strengths among many other factors. With a strong adhesive on both sides, double-sided tape is just as reliable as a nail in the wall but has the versatility and convenience of regular duct tape. If you’re worried about whether or not it’s strong enough, don’t worry, some heavy-duty double-sided tapes can safely hold up to 30 pounds of weight. It’s just as effective at holding things up on your wall and is way quicker to work with as compared to hammering nails or drilling holes, but without the issue of possibly damaging your walls. With regular glue being too messy, and drilling (or nails) being too harmful to your walls, there’s only one alternative that makes the most sense for day-to-day DIY projects - and that’s double-sided tape. ![]() It is a combo of things actually that no other camera manufacturer has been able to accomplish as of yet besides Olympus. I have finally figured it out though.as to just what that special thing is. I have said it a million times about these Olympus bodies but they have something about them that are special. The E-M5, E-P5, E-M1 are stellar, and I mean that 100%. ALL of these cameras have been wonderful to use and to shoot but especially these new camera bodies Olympus have been releasing over the past couple of years. The PEN series.yes, I have shot with them all (The E-P5 is the best of PEN’s) and of course the OM-D series all the way to the top of the Micro 4/3 heap with the Professional E-M1. From the OM film cameras to the 1st real flagship E-1 digital back in the day. “Orgasim Clinic” – what happens in vegas stays in vegas… Basically, when using the 12-40 and the E-M10 I would highly recommend the accessory grip as the body is a little small for the Zoom as is. It did have some faults but only in the handling. The Olympus was so much fun, so fast and so GOOD that I did not want to stop using it! Seriously. I did end up bringing along my Leica M 240 and 50 1.5 Nokton but only fired off 6 shots. So one morning Debby and I took a 4 hour drive to Las Vegas and decided to just walk around and shoot the scenery. It is IMO, the only way to test a camera for what it is meant to be used for. I always prefer real world use of a camera and have been doing these types of reviews and write ups for almost 6 years now. Please note! This is not a normal tech “review” but it will be more of my thoughts using this camera over a weekend along with the photos I was able to casually snap. No need to worry or stress over lenses to use or take with. WHY? Well, I have not yet reviewed this lens and figured it would be a nice test of the camera and lens. I ended up deciding to just shoot this camera over a weekend with the Olympus 12-40 Pro Zoom. It surprised me really as I was expecting it to be a little “laggy” seeing that it is a “mini”model that is cheaper. It felt solid, booted up quickly and had that blazing Olympus AF speed I have come to love and trust. When I opened it up I was impressed with the look, the style and the design.which is basically just like the OM-D E-M5, just in a mini format. ![]() So one day I received a UPS box from the wonderful B&H Photo who sent me the little black Olympus E-M10 to check out and review. This lens is truly a pro zoom with a constant f/2.8 aperture across the range.ĪLL images in this review are JPEGS shot with the E-M10 and 12-40 2.8 Lens! The IQ is stellar. The color reproduction is rich. The E-M10 and 12-40 2.8 – This combo is lightning fast, sharp, amazing prime IQ and the very 1st zoom I have ever really wanted to buy in the mirror less world. “well, if it is as great as the E-M5 in use, it will be a BARGAIN of epic proportions”! Then I realized that it had even better performance than the E-M5 and equaled the E-M1 for IQ and Af Speed. Then I saw the cost of only $699 and thought. ![]() I glossed over it and while it looked cool I had doubts about why anyone would want it over an E-m5. Well, that was the question I had when it was announced. These are three Micro 4/3 mirrorless models that are truly state of the art and can easily provide anyone with gorgeous quality photos while offering speed, build and features that would make any shutter bug happy and many other mirror less camera companies a little nervous. The PEN E-P5 is fantastic and beautiful and one of my faves of all time in Micro 4/3. I mean, was Olympus going the way of Panasonic who in the past released cheap, dumbed down tiny bodies just to make a quick sale and find a market for their camera bodies? Let’s face it, the OM-D E-M5 is stellar. With Olympus continually being on top of their own mirrorless camera game I admit…I was a bit hesitant when the E-M10 was announced. The Olympus E-M10 and 12-40 f/2.8 Pro Zoom Mini Review 8/30/2023 0 Comments Purpose of western blot analysis![]() Usually the tissue needs to be broken down by blending, homogenization, or sonication. tissue, cells, or other solution, which is going to be analyzed. The first step of a WB is to prepare the sample, e.g. However, WB is a very common method and almost all available commercial antibodies have been validated using this method. The result form the WB is not always easy to interpret as the size of the protein may vary from the theoretical weight due to posttranslational modifications, such as glycosylation, or interactions with other proteins. Moreover, the specificity of the binding to the target and a low cross reactivity are important features as well. The outcome of a WB experiment depends on three important factors the ability of the antibody to bind a specific protein, the strength of the interaction, and the concentration of the protein of interest itself. In addition, the non-linear relation of the generated signal across the concentration range of the samples is also an aspect of consideration when interpreting the results. When analyzing the results, variations between lanes regarding loading and transfer rates between blots, must be taken into consideration. The setup of the experiment can be varied in many ways to best suit the specific inquiry. The separation on the gel is not only due to size but also to some extent depending on the molecular charge, hydrophobic regions, and degree of denaturation. An image is taken of the membrane and the result is analyzed.īy adding a separate marker solution to one of the wells in the gel, it is possible to estimate the size of the protein in addition to the antibody interactions that are used to verify the specific protein. To visualize the protein of interest the membrane is commonly first probed using a primary protein-specific antibody followed by a labeled secondary antibody used for detection. After the transfer, the membrane is blocked in order to prevent unwanted membrane-protein interaction in the following steps. In order to further analyze the proteins, they are transferred onto a membrane in a procedure called blotting. Samples are prepared and loaded on to a gel and during the electrophoresis the negatively charged proteins move toward the positively charged anode. The setup consists of a standard set of seven steps, Figure 1.įigure 1. Towbin et al described electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets where the original gel pattern was accurately obtained. Towbin et al in 1979 ( Towbin, Staehelin, & Gordon, 1979) and two years later given its name by W. ![]() It is built on a technique that involves transferring, also known as blotting, proteins separated by electrophoresis from the gel to a membrane where they can be visualized specifically. Western Blot (WB) is a common method to detect and analyze proteins. |
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